Partial Purification and Some Properties of Bovine Milk Alkaline Phosphatase
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چکیده
منابع مشابه
Purification and properties of bovine synovial fluid alkaline phosphatase.
Alkaline phosphatase from bovine synovial fluid was purified 2300-fold. A molecular weight of 72,300 was determined from sucrose density gradient studies. The following monoesters were hydrolyzed by the enzyme: P-glycerophosphate, galactosamine 6-phosphate, glucosamice 6-phosphate, glucose 6-phosphate, o-phospho-L-serine, o-carboxyphenyl phosphate, phenyl phosphate, and p-nitrophenyl phosphate....
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in this thesis, at first we investigate the bounded inverse theorem on fuzzy normed linear spaces and study the set of all compact operators on these spaces. then we introduce the notions of fuzzy boundedness and investigate a new norm operators and the relationship between continuity and boundedness. and, we show that the space of all fuzzy bounded operators is complete. finally, we define...
15 صفحه اولPartial . Purification and Some Properties
Two polyphenoloxidases (PPO ] and PPO III, EC 1,10.3,1) were extracted and partially purified from sago palm pith by hydroxylapatite chromatography, DEAE-cellulose chromategraphy and gel filtration. Both purified isozymes gave a single activity band on polyacrylamide gel electrophoresis. The rno]ecular weights of both enzymes were estimated to be 40,OOO. Thev had the same pH optima of 6,5 but ...
متن کاملAlkaline Phosphatase of Milk
inor i8obutanol. Removal of the lipid is accompanied by simultaneous release of the enzyme into true solution. No other organic solvent tested was effective in releasing the bound phosphatase, indicating a unique action of butanol. I wish to thank Dr M. Dixon, F.R.S., for his encouraging interest and advice during this work. This work was carried out while holding a Travelling Scholarship award...
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1. The phosphorylation of milk alkaline phosphatase was studied under various conditions: maximum incorporation occurred at pH5.0 and 50% incorporation at pH6.6-7.0. 2. The phosphorylation was shown to be specific and the results suggest that the active centre of the enzyme is involved in the process. 3. Phosphoryl-enzyme is rapidly hydrolysed at alkaline pH. at pH7.0 the results suggest that a...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 1980
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.51.501